› 1ia8

X-ray diffraction
1.7Å resolution

THE 1.7 A CRYSTAL STRUCTURE OF HUMAN CELL CYCLE CHECKPOINT KINASE CHK1

Released:
DOI: 10.2210/pdb1ia8/pdb
PDB entry 1ia8 coloured by chain, front view.
PDB entry 1ia8 coloured by chain, side view.
PDB entry 1ia8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The 1.7 A crystal structure of human cell cycle checkpoint kinase Chk1: implications for Chk1 regulation.
Chen P, Luo C, Deng Y, Ryan K, Register J, Margosiak S, Tempczyk-Russell A, Nguyen B, Myers P, Lundgren K, Kan CC, O'Connor PM
Cell 100 681-92 (2000)
PMID: 10761933

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127093 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase Chk1 Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 33.04 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O14757 (Residues: 1-289; Coverage: 61%)
Gene names: CHEK1, CHK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 45.2Å b: 65.7Å c: 58.1Å
α: 90° β: 93.9° γ: 90°
R-values:
R R work R free
0.216 0.216 0.235
Expression system: Spodoptera frugiperda

Quick links

Citations

17 review citations

Regulation of protein kinases; controlling activity through activation segment conformation.
Nolen et al. (2004)
16 more

24 mentions without citation

Substrate and docking interactions in serine/threonine protein kinases.
Goldsmith et al. (2007)
23 more