› 1joc

X-ray diffraction
2.2Å resolution

EEA1 homodimer of C-terminal FYVE domain bound to inositol 1,3-diphosphate

Released:
DOI: 10.2210/pdb1joc/pdb
PDB entry 1joc coloured by chain, front view.
PDB entry 1joc coloured by chain, side view.
PDB entry 1joc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Multivalent endosome targeting by homodimeric EEA1.
Dumas JJ, Merithew E, Sudharshan E, Rajamani D, Hayes S, Lawe D, Corvera S, Lambright DG
Mol Cell 8 947-58 (2001)
PMID: 11741531

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172186 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Early endosome antigen 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 125 amino acids
Theoretical weight: 14.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15075 (Residues: 1287-1324, 1326-1411; Coverage: 9%)
Gene names: EEA1, ZFYVE2
Sequence domains: FYVE zinc finger
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand ITP 2 x ITP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 36.528Å b: 85.096Å c: 88.036Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.281
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

38 review citations

Endocytic recycling.
Maxfield et al. (2004)
37 more

38 mentions without citation

The ESCRT complexes.
Hurley JH. (2010)
37 more