› 1u04

X-ray diffraction
2.25Å resolution

Crystal structure of full length Argonaute from Pyrococcus furiosus

Released:
DOI: 10.2210/pdb1u04/pdb
PDB entry 1u04 coloured by chain, front view.
PDB entry 1u04 coloured by chain, side view.
PDB entry 1u04 coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
Crystal structure of Argonaute and its implications for RISC slicer activity.
Song JJ, Smith SK, Hannon GJ, Joshua-Tor L
Science 305 1434-7 (2004)
PMID: 15284453

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186456 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein argonaute Chain: A
Molecule details ›
Chain: A
Length: 771 amino acids
Theoretical weight: 91.44 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U3D2 (Residues: 1-770; Coverage: 100%)
Gene names: PF0537, ago
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25, null
Spacegroup: P21
Unit cell:
a: 69.726Å b: 104.188Å c: 74.015Å
α: 90° β: 102.83° γ: 90°
R-values:
R R work R free
0.227 0.227 0.258
Expression system: Escherichia coli

Quick links

Citations

297 review citations

MicroRNAs: target recognition and regulatory functions.
Bartel DP. (2009)
296 more

19 mentions without citation

RNA-binding proteins: modular design for efficient function.
Lunde et al. (2007)
18 more