› 2hbj

X-ray diffraction
2.1Å resolution

Structure of the yeast nuclear exosome component, Rrp6p, reveals an interplay between the active site and the HRDC domain

Released:
DOI: 10.2210/pdb2hbj/pdb
PDB entry 2hbj coloured by chain, front view.
PDB entry 2hbj coloured by chain, side view.
PDB entry 2hbj coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of the nuclear exosome component Rrp6p reveals an interplay between the active site and the HRDC domain.
Midtgaard SF, Assenholt J, Jonstrup AT, Van LB, Jensen TH, Brodersen DE
Proc Natl Acad Sci U S A 103 11898-903 (2006)
PMID: 16882719

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exosome complex exonuclease RRP6 Chain: A
Molecule details ›
Chain: A
Length: 410 amino acids
Theoretical weight: 47.68 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12149 (Residues: 129-536; Coverage: 56%)
Gene names: RRP6, UNC733, YOR001W
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A, EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P3121
Unit cell:
a: 110.135Å b: 110.135Å c: 80.169Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.232 0.292
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
18 more

4 mentions without citation

A strategy for dissecting the architectures of native macromolecular assemblies.
Shi et al. (2015)
3 more