› 2i5s

X-ray diffraction
1.9Å resolution

Crystal structure of onconase with bound nucleic acid

Released:
DOI: 10.2210/pdb2i5s/pdb
PDB entry 2i5s coloured by chain, front view.
PDB entry 2i5s coloured by chain, side view.
PDB entry 2i5s coloured by chain, top view.
Source organism: Lithobates pipiens
Primary publication:
Structural basis for catalysis by onconase.
Lee JE, Bae E, Bingman CA, Phillips GN, Raines RT
J Mol Biol 375 165-77 (2008)
PMID: 18001769

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115384 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Protein P-30 Chain: X
Molecule details ›
Chain: X
Length: 104 amino acids
Theoretical weight: 11.85 KDa
Source organism: Lithobates pipiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22069 (Residues: 1-104; Coverage: 100%)
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain
5'-D(*A*(DU)P*GP*A)-3' Chain: A
Molecule details ›
Chain: A
Length: 4 nucleotides
Theoretical weight: 1.2 KDa
Source organism: Lithobates pipiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P21212
Unit cell:
a: 129.229Å b: 26.101Å c: 32.486Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.178 0.24
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Ribonucleases as potential modalities in anticancer therapy.
Ardelt et al. (2009)
3 more

5 mentions without citation

Protein crystallography for non-crystallographers, or how to get the best (but not more) from published macromolecular structures.
Wlodawer et al. (2008)
4 more