› 2lrp

Solution NMR

Solution structure, dynamics and binding studies of CtCBM11

Released:
DOI: 10.2210/pdb2lrp/pdb
PDB entry 2lrp coloured by chain, ensemble of 20 models, front view.
PDB entry 2lrp coloured by chain, ensemble of 20 models, side view.
PDB entry 2lrp coloured by chain, ensemble of 20 models, top view.
Source organism: Acetivibrio thermocellus ATCC 27405
Primary publication:
Solution structure, dynamics and binding studies of a family 11 carbohydrate-binding module from Clostridium thermocellum (CtCBM11).
Viegas A, Sardinha J, Freire F, Duarte DF, Carvalho AL, Fontes CM, Romão MJ, Macedo AL, Cabrita EJ
Biochem J 451 289-300 (2013)
PMID: 23356867

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147658 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase H Chain: A
Molecule details ›
Chain: A
Length: 172 amino acids
Theoretical weight: 18.96 KDa
Source organism: Acetivibrio thermocellus ATCC 27405
Expression system: Escherichia coli
UniProt:
  • Canonical: P16218 (Residues: 655-821; Coverage: 20%)
Gene names: Cthe_1472, celH
Sequence domains: Carbohydrate binding domain (family 11)
Structure domains: Galactose-binding lectin

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 81%
Refinement method: molecular dynamics
Expression system: Escherichia coli

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Citations

9 citation in other articles

An unusual mode of galactose recognition by a family 32 carbohydrate-binding module.
Grondin et al. (2014)
8 more