› 2pol

X-ray diffraction
2.5Å resolution

THREE-DIMENSIONAL STRUCTURE OF THE BETA SUBUNIT OF ESCHERICHIA COLI DNA POLYMERASE III HOLOENZYME: A SLIDING DNA CLAMP

Released:
DOI: 10.2210/pdb2pol/pdb
PDB entry 2pol coloured by chain, front view.
PDB entry 2pol coloured by chain, side view.
PDB entry 2pol coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Three-dimensional structure of the beta subunit of E. coli DNA polymerase III holoenzyme: a sliding DNA clamp.
Kong XP, Onrust R, O'Donnell M, Kuriyan J
Cell 69 425-37 (1992)
PMID: 1349852

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141822 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta sliding clamp Chains: A, B
Molecule details ›
Chains: A, B
Length: 366 amino acids
Theoretical weight: 40.63 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P0A988 (Residues: 1-366; Coverage: 100%)
Gene names: JW3678, b3701, dnaN
Sequence domains:
Structure domains: DNA Polymerase III, subunit A, domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 80.61Å b: 68.35Å c: 82.35Å
α: 90° β: 114.26° γ: 90°
R-values:
R R work R free
0.184 0.184 not available
Expression system: Not provided

Quick links

Citations

87 review citations

The DNA replication fork in eukaryotic cells.
Waga et al. (1998)
86 more

43 mentions without citation

Replication clamps and clamp loaders.
Hedglin et al. (2013)
42 more