› 2wwp

X-ray diffraction
2Å resolution

Crystal structure of the human lipocalin-type prostaglandin D synthase

Released:
DOI: 10.2210/pdb2wwp/pdb
PDB entry 2wwp coloured by chain, front view.
PDB entry 2wwp coloured by chain, side view.
PDB entry 2wwp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and dynamic insights into substrate binding and catalysis of human lipocalin prostaglandin D synthase.
Lim SM, Chen D, Teo H, Roos A, Jansson AE, Nyman T, Trésaugues L, Pervushin K, Nordlund P
J Lipid Res 54 1630-1643 (2013)
PMID: 23526831

Function and Biology Details

Reaction catalysed: 
(5Z,13E,15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E,15S)-9-alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154342 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Prostaglandin-H2 D-isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 176 amino acids
Theoretical weight: 19.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P41222 (Residues: 23-190; Coverage: 100%)
Gene names: PDS, PTGDS
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand SCN 2 x SCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 36.4Å b: 56.59Å c: 73.46Å
α: 90° β: 90.15° γ: 90°
R-values:
R R work R free
0.21 0.208 0.246
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Molecular mechanisms of amyloid disaggregation.
Low et al. (2022)
9 other articles

3 mentions without citation

The major cockroach allergen Bla g 4 binds tyramine and octopamine.
Offermann et al. (2014)
2 more