› 3i3j

X-ray diffraction
2.33Å resolution

Crystal Structure of the Bromodomain of Human EP300

Released:
DOI: 10.2210/pdb3i3j/pdb
PDB entry 3i3j coloured by chain, front view.
PDB entry 3i3j coloured by chain, side view.
PDB entry 3i3j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Histone recognition and large-scale structural analysis of the human bromodomain family.
Filippakopoulos P, Picaud S, Mangos M, Keates T, Lambert JP, Barsyte-Lovejoy D, Felletar I, Volkmer R, Müller S, Pawson T, Gingras AC, Arrowsmith CH, Knapp S
Cell 149 214-31 (2012)
PMID: 22464331

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170794 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone acetyltransferase p300 Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 124 amino acids
Theoretical weight: 14.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09472 (Residues: 1040-1161; Coverage: 5%)
Gene names: EP300, P300
Sequence domains: Bromodomain
Structure domains: Bromodomain-like

Ligands and Environments

3 bound ligands:
Ligand CL 2 x CL
Ligand PEG 2 x PEG
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 64.899Å b: 86.186Å c: 149.897Å
α: 90° β: 96.98° γ: 90°
R-values:
R R work R free
0.231 0.229 0.275
Expression system: Escherichia coli

Quick links

Citations

244 review citations

Modification of enhancer chromatin: what, how, and why?
Calo et al. (2013)
243 more

6 mentions without citation

Protein lysine acetylation by p300/CBP.
Dancy et al. (2015)
5 more