3id6

X-ray diffraction
2.6Å resolution

Crystal structure of Sulfolobus solfataricus Nop5 (1-262) and fibrillarin complex

Released:
Source organism: Saccharolobus solfataricus
Primary publication:
Structural organization of box C/D RNA-guided RNA methyltransferase.
Proc Natl Acad Sci U S A 106 13808-13 (2009)
PMID: 19666563

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-157642 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nop domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 268 amino acids
Theoretical weight: 31.14 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97ZH3 (Residues: 1-262; Coverage: 64%)
Gene name: SSO0939
Sequence domains:
Structure domains:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase Chain: C
Molecule details ›
Chain: C
Length: 232 amino acids
Theoretical weight: 26.44 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: P58032 (Residues: 1-232; Coverage: 100%)
Gene names: C33_014, SSO0940, flpA
Sequence domains: Fibrillarin
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2221
Unit cell:
a: 75.517Å b: 182.364Å c: 79.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.232 0.285
Expression system: Escherichia coli