› 3l5l

X-ray diffraction
1.03Å resolution

Xenobiotic Reductase A - oxidized

Released:
DOI: 10.2210/pdb3l5l/pdb
PDB entry 3l5l coloured by chain, front view.
PDB entry 3l5l coloured by chain, side view.
PDB entry 3l5l coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Cysteine as a modulator residue in the active site of xenobiotic reductase A: a structural, thermodynamic and kinetic study.
Spiegelhauer O, Mende S, Dickert F, Knauer SH, Ullmann GM, Dobbek H
J Mol Biol 398 66-82 (2010)
PMID: 20206186

Function and Biology Details

Reaction catalysed: 
NADPH + acceptor = NADP(+) + reduced acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174908 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 363 amino acids
Theoretical weight: 39.91 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3ZDM6 (Residues: 1-363; Coverage: 100%)
Gene name: xenA
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
2 bound ligands:
Ligand SO4 7 x SO4
Ligand BU3 3 x BU3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: I222
Unit cell:
a: 57.893Å b: 83.416Å c: 156.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.13 0.129 0.148
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Microbial 2,4,6-trinitrotoluene degradation: could we learn from (bio)chemistry for bioremediation and vice versa?
Stenuit et al. (2010)
1 more

3 mentions without citation

Mapping between EQ-5D-3L and EQ-5D-5L: A survey experiment on the validity of multi-instrument data.
Hernández-Alava et al. (2022)
2 more