› 3n7n

X-ray diffraction
3.9Å resolution

Structure of Csm1/Lrs4 complex

Released:
DOI: 10.2210/pdb3n7n/pdb
PDB entry 3n7n coloured by chain, front view.
PDB entry 3n7n coloured by chain, side view.
PDB entry 3n7n coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The monopolin complex crosslinks kinetochore components to regulate chromosome-microtubule attachments.
Corbett KD, Yip CK, Ee LS, Walz T, Amon A, Harrison SC
Cell 142 556-67 (2010)
PMID: 20723757

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150542 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Monopolin complex subunit CSM1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 190 amino acids
Theoretical weight: 21.78 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25651 (Residues: 1-190; Coverage: 100%)
Gene names: CSM1, SPO86, YCR086W, YCR86W
Sequence domains:
Structure domains:
Monopolin complex subunit LRS4 Chains: E, F
Molecule details ›
Chains: E, F
Length: 95 amino acids
Theoretical weight: 11.24 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q04087 (Residues: 1-102; Coverage: 27%)
Gene names: D9461.25, LRS4, YDR439W
Sequence domains: Monopolin complex subunit LRS4

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P321
Unit cell:
a: 152.624Å b: 152.624Å c: 118.794Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.333 0.333 0.355
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

Causes and consequences of aneuploidy in cancer.
Gordon et al. (2012)
26 more

2 mentions without citation

The monopolin complex crosslinks kinetochore components to regulate chromosome-microtubule attachments.
Corbett et al. (2010)
1 more