› 4daj

X-ray diffraction
3.4Å resolution

Structure of the M3 Muscarinic Acetylcholine Receptor

Released:
DOI: 10.2210/pdb4daj/pdb
PDB entry 4daj coloured by chain, front view.
PDB entry 4daj coloured by chain, side view.
PDB entry 4daj coloured by chain, top view.
Source organisms:
Primary publication:
Structure and dynamics of the M3 muscarinic acetylcholine receptor.
Kruse AC, Hu J, Pan AC, Arlow DH, Rosenbaum DM, Rosemond E, Green HF, Liu T, Chae PS, Dror RO, Shaw DE, Weis WI, Wess J, Kobilka BK
Nature 482 552-6 (2012)
PMID: 22358844

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-132957 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin; Muscarinic acetylcholine receptor M3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 479 amino acids
Theoretical weight: 54.99 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08483 (Residues: 57-259, 482-589; Coverage: 53%)
  • Canonical: P00720 (Residues: 1-161; Coverage: 98%)
Gene names: Chrm-3, Chrm3, E
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 0HK 4 x 0HK
Ligand PO4 10 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P1
Unit cell:
a: 54.77Å b: 61.31Å c: 176.91Å
α: 85.87° β: 89.9° γ: 84.9°
R-values:
R R work R free
0.254 0.251 0.303
Expression system: Spodoptera frugiperda

Quick links

Citations

121 review citations

Molecular signatures of G-protein-coupled receptors.
Venkatakrishnan et al. (2013)
120 more

104 mentions without citation

The GPCR Network: a large-scale collaboration to determine human GPCR structure and function.
Stevens et al. (2013)
103 more