› 4ihb

X-ray diffraction
2.04Å resolution

X-RAY STRUCTURE OF THE canonical C2A DOMAIN FROM HUMAN DYSFERLIN

Released:
DOI: 10.2210/pdb4ihb/pdb
PDB entry 4ihb coloured by chain, front view.
PDB entry 4ihb coloured by chain, side view.
PDB entry 4ihb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Alternate splicing of dysferlin C2A confers Ca²⁺-dependent and Ca²⁺-independent binding for membrane repair.
Fuson K, Rice A, Mahling R, Snow A, Nayak K, Shanbhogue P, Meyer AG, Redpath GM, Hinderliter A, Cooper ST, Sutton RB
Structure 22 104-15 (2014)
PMID: 24239457

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-131173 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dysferlin Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 133 amino acids
Theoretical weight: 14.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75923 (Residues: 1-124; Coverage: 6%)
Gene names: DYSF, FER1L1
Sequence domains: C2 domain
Structure domains: C2 domain

Ligands and Environments

2 bound ligands:
Ligand FMT 39 x FMT
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU, SSRL BEAMLINE BL7-1
Unit cell:
a: 102.413Å b: 70.67Å c: 118.31Å
α: 90° β: 113.39° γ: 90°
R-values:
R R work R free
0.204 0.2 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Auditory neuropathy--neural and synaptic mechanisms.
Moser et al. (2016)
8 more

8 mentions without citation

Otoferlin acts as a Ca2+ sensor for vesicle fusion and vesicle pool replenishment at auditory hair cell ribbon synapses.
Michalski et al. (2017)
7 more