› 5jnp

X-ray diffraction
2.4Å resolution

Crystal structure of a rice (Oryza Sativa) cellulose synthase plant conserved region (P-CR)

Released:
DOI: 10.2210/pdb5jnp/pdb
PDB entry 5jnp coloured by chain, front view.
PDB entry 5jnp coloured by chain, side view.
PDB entry 5jnp coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Rice Cellulose SynthaseA8 Plant-Conserved Region Is a Coiled-Coil at the Catalytic Core Entrance.
Rushton PS, Olek AT, Makowski L, Badger J, Steussy CN, Carpita NC, Stauffacher CV
Plant Physiol 173 482-494 (2017)
PMID: 27879387

Function and Biology Details

Reaction catalysed: 
UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-183080 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable cellulose synthase A catalytic subunit 8 [UDP-forming] Chains: A, B
Molecule details ›
Chains: A, B
Length: 128 amino acids
Theoretical weight: 15.01 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: Q84ZN6 (Residues: 398-523; Coverage: 12%)
Gene names: CESA8, LOC_Os07g10770, OJ1136_A05.10, OJ1559_F09.120, Os07g0208500, OsJ_022567

Ligands and Environments

3 bound ligands:
Ligand FLC 2 x FLC
Ligand GOL 2 x GOL
Ligand PO4 2 x PO4
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: F23
Unit cell:
a: 150.817Å b: 150.817Å c: 150.817Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.24 0.272
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Cellulose synthase complex organization and cellulose microfibril structure.
Turner et al. (2018)
1 more

2 mentions without citation

Computational approach to understand molecular mechanism involved in BPH resistance in Bt- rice plant.
Gupta et al. (2019)
1 more