› 5a6w

X-ray diffraction
1.6Å resolution

Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikD with the HMA domain of Pikp1 from rice (Oryza sativa)

Released:
DOI: 10.2210/pdb5a6w/pdb
PDB entry 5a6w coloured by chain, front view.
PDB entry 5a6w coloured by chain, side view.
PDB entry 5a6w coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of pathogen recognition by an integrated HMA domain in a plant NLR immune receptor.
Maqbool A, Saitoh H, Franceschetti M, Stevenson CE, Uemura A, Kanzaki H, Kamoun S, Terauchi R, Banfield MJ
Elife 4 (2015)
PMID: 26304198

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111281 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
HMA domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 75 amino acids
Theoretical weight: 7.82 KDa
Source organism: Oryza sativa
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: E9KPB5 (Residues: 186-258; Coverage: 6%)
Gene name: Pikp-1
Structure domains: Alpha-Beta Plaits
AVR-Pik protein Chain: C
Molecule details ›
Chain: C
Length: 93 amino acids
Theoretical weight: 10.83 KDa
Source organism: Pyricularia oryzae
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: C4B8B8 (Residues: 22-113; Coverage: 100%)
Gene names: AVR-Pik, AvrPik, Pikm, Pikp

Ligands and Environments

2 bound ligands:
Ligand EDO 4 x EDO
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P41212
Unit cell:
a: 118.41Å b: 118.41Å c: 35.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.178 0.205
Expression system: Escherichia coli K-12

Quick links

Citations

32 review citations

Defended to the Nines: 25 Years of Resistance Gene Cloning Identifies Nine Mechanisms for R Protein Function.
Kourelis et al. (2018)
31 more

11 mentions without citation

Effectors of Filamentous Plant Pathogens: Commonalities amid Diversity.
Franceschetti et al. (2017)
10 more