Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 8r6u
Q-score: 0.565
Williams HM, Thorkelsson SR, Vogel D, Busch C, Milewski M, Cusack S, Grunewald K, Quemin ERJ, Rosenthal M
BiorXiv (2023) [ DOI: doi:10.1101/2023.11.29.569195 ]
- Sftsv l protein in complex with 5' and 3' rna, as well as rna acting as a primer. (240 kDa, Complex from SFTS virus AH12)
- Rna (5'-r(p*cp*up*gp*gp*gp*cp*gp*gp*up*cp*up*up*up*gp*up*gp*u)-3') (8 kDa, RNA from SFTS virus AH12)
- Rna-directed rna polymerase l (235 kDa, Protein from SFTS virus AH12)
- Magnesium ion (24 Da, Ligand)
- Rna primer tail-end (5 kDa, RNA from SFTS virus AH12)
- Rna primer (6 kDa, RNA from SFTS virus AH12)
Resolution: 3.35 Å
EM Method: Single-particle
Fitted PDBs: 8r6w
Q-score: 0.44
Williams HM, Thorkelsson SR, Vogel D, Busch C, Milewski M, Cusack S, Grunewald K, Quemin ERJ, Rosenthal M
BiorXiv (2023) [ DOI: doi:10.1101/2023.11.29.569195 ]
- Sftsv l protein in complex with 5' and 3' rna, as well as rna acting as a primer. (240 kDa, Complex from SFTS virus AH12)
- Rna (5'-r(p*cp*up*gp*gp*gp*cp*gp*gp*up*cp*up*up*up*gp*up*gp*u)-3') (8 kDa, RNA from SFTS virus AH12)
- Rna-directed rna polymerase l (235 kDa, Protein from SFTS virus AH12)
- Rna (5'-r(*(m7g)*ap*ap*a)-3') (5 kDa, RNA from SFTS virus AH12)
- Rna (5'-r(*ap*cp*ap*c)-3') (5 kDa, RNA from SFTS virus AH12)
- Rna (5'-r(*ap*cp*ap*cp*ap*gp*ap*gp*ap*cp*gp*cp*cp*cp*ap*g)-3') (6 kDa, RNA from SFTS virus AH12)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8r6y
Q-score: 0.456
Williams HM, Thorkelsson SR, Vogel D, Busch C, Milewski M, Cusack S, Grunewald K, Quemin ERJ, Rosenthal M
BiorXiv (2023) [ DOI: doi:10.1101/2023.11.29.569195 ]
- Sftsv l protein in complex with 5' and 3' rna, as well as rna acting as a primer. (240 kDa, Complex from SFTS virus AH12)
- Rna-directed rna polymerase l (235 kDa, Protein from SFTS virus AH12)
- Rna (5'-r(*(m7g)*ap*ap*a)-3') (5 kDa, RNA from SFTS virus AH12)
- Rna (5'-r(p*ap*cp*a)-3') (918 Da, RNA from SFTS virus AH12)
- Rna (5'-r(p*cp*up*gp*gp*gp*cp*gp*gp*up*ap*ap*ap*up*gp*u)-3') (6 kDa, RNA from SFTS virus AH12)
- Magnesium ion (24 Da, Ligand)
- 5'-o-[(s)-hydroxy{[(s)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]uridine (483 Da, Ligand)
- Rna (5'-r(*ap*cp*ap*cp*ap*gp*ap*gp*ap*cp*gp*cp*cp*cp*ap*g)-3') (6 kDa, RNA from SFTS virus AH12)
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 8w20
Q-score: 0.295
Park YJ, Veesler D, Mougous JD
Nature (2024) [ DOI: doi:10.1038/s41586-024-07298-z ]
- Umb1 umbrella toxin particle (Complex from Streptomyces coelicolor A3(2))
- Intein c-terminal splicing domain-containing protein (142 kDa, Protein from Streptomyces coelicolor A3(2))
- Secreted protein (17 kDa, Protein from Streptomyces coelicolor A3(2))
- Secreted esterase (54 kDa, Protein from Streptomyces coelicolor A3(2))
Umb1 umbrella toxin particle (local refinement of UmbB1 bound ALF of UmbC1 and UmbA1)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 8w22
Q-score: 0.386
Park YJ, Veesler D, Mougous JD
Nature (2024) [ DOI: doi:10.1038/s41586-024-07298-z ]
- Umb1 umbrella toxin particle (local refinement of umbb1 bound alf of umbc1 and umba1) (Complex from Streptomyces coelicolor A3(2))
- Intein c-terminal splicing domain-containing protein (142 kDa, Protein from Streptomyces coelicolor A3(2))
- Secreted protein (17 kDa, Protein from Streptomyces coelicolor A3(2))
- Secreted esterase (54 kDa, Protein from Streptomyces coelicolor A3(2))
To Be Published
Image sets:
Structure of the formin Cdc12 bound to the barbed end of phalloidin-stabilized F-actin.
Resolution: 3.56 Å
EM Method: Single-particle
Fitted PDBs: 8rtt
Q-score: 0.444
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
To Be Published
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Dimeric fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin filament (Complex from Homo sapiens)
- Cell division control protein 12 (48 kDa, Protein from Schizosaccharomyces pombe)
- Magnesium ion (24 Da, Ligand)
- Phalloidin. (Complex from Amanita phalloides)
- Phosphate ion (94 Da, Ligand)
- Complex of the dimeric fh2 domain of s. pombe cdc12 bound to the barbed end of phalloidin stabilized f-actin. (Complex)
Cryo-EM reconstruction of the formin Cdc12 bound to the barbed end of F-actin (without phalloidin)
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Actin filament (Complex)
- Dimeric fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
- Complex of the dimeric fh2 domain of s. pombe cdc12 bound to the barbed end of f-actin. (Complex from Homo sapiens)
- Human cytoplasmic beta-actin (Protein from Homo sapiens)
- S. pombe cdc12 (Protein from Schizosaccharomyces pombe)
Resolution: 6.25 Å
EM Method: Single-particle
Fitted PDBs: 8rty
Q-score: 0.169
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin-formin-profilin ring complex: the phalloidin-stabilized f-actin barbed end bound by dimeric-cdc12 and profilin-s71m. (Complex)
- Profilin-1-s29c/s71m (Complex from Homo sapiens)
- Dimeric fh1fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
- Phalloidin (Complex from Amanita phalloides)
- Methylated-dna--protein-cysteine methyltransferase,cell division control protein 12 (77 kDa, Protein from Schizosaccharomyces pombe)
- Phalloidin (amanita phalloides) (808 Da, Protein from Amanita phalloides)
- Actin filament (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Profilin-1 (15 kDa, Protein from Homo sapiens)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Phosphate ion (94 Da, Ligand)
Structure of the undecorated barbed end of F-actin.
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8ru0
Q-score: 0.542
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin filament (Complex)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Complex of the actin subunits that form the barbed end of actin filaments. (Complex from Oryctolagus cuniculus)
- Phosphate ion (94 Da, Ligand)