Cryo-EM structure of human Needle/NAIP/NLRC4 (R288A)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8fvu
Q-score: 0.412
Matico RE, Yu X, Miller R, Somani S, Ricketts MD, Kumar N, Steele RA, Medley Q, Berger S, Faustin B, Sharma S
Nat Struct Mol Biol (2024) 31 pp. 82-91 [ Pubmed: 38177670 DOI: doi:10.1038/s41594-023-01143-z ]
- Nlr family card domain-containing protein 4 (116 kDa, Protein from Homo sapiens)
- Baculoviral iap repeat-containing protein 1 (160 kDa, Protein from Homo sapiens)
- Human naip and nlrc4 (Complex from Homo sapiens)
- Lethal factor,type iii secretion system protein (42 kDa, Protein from Burkholderia)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Burkholderia needle (chimeric with an anthrax toxin lethal factor tag) (Complex from Bacillus anthracis)
- The complex of needle, human naip and nlrc4 (Complex)
- Zinc ion (65 Da, Ligand)
cryoEM map of PA D425A mutant at pH 5.5 D7 symmetry class 1
Scott H, Huang W, Andra K, Mamillapalli S, Gonti S, Day A, Zhang K, Mehzabeen N, Battaile KP, Raju A, Lovell S, Bann JG, Taylor DJ
J Mol Biol (2022) 434 pp. 167548-167548 [ Pubmed: 35304125 DOI: doi:10.1016/j.jmb.2022.167548 ]
Scott H, Huang W, Andra K, Mamillapalli S, Gonti S, Day A, Zhang K, Mehzabeen N, Battaile KP, Raju A, Lovell S, Bann JG, Taylor DJ
J Mol Biol (2022) 434 pp. 167548-167548 [ Pubmed: 35304125 DOI: doi:10.1016/j.jmb.2022.167548 ]
heptamer of D425A mutant at pH 5.5
Scott H, Huang W, Andra K, Mamillapalli S, Gonti S, Day A, Zhang K, Mehzabeen N, Battaile KP, Raju A, Lovell S, Bann JG, Taylor DJ
J Mol Biol (2022) 434 pp. 167548-167548 [ Pubmed: 35304125 DOI: doi:10.1016/j.jmb.2022.167548 ]
Structure of the P. aeruginosa ExoY-F-actin complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7p1g
Q-score: 0.46
Belyy A, Merino F, Mechold U, Raunser S
Nat Commun (2021) 12 pp. 6628-6628 [ DOI: doi:10.1038/s41467-021-26889-2 Pubmed: 34785651 ]
- Phalloidin (808 Da, Protein from Amanita phalloides)
- The complex of p. aeruginosa exoy with f-actin (Complex)
- 3'-deoxy-guanosine-5'-triphosphate (507 Da, Ligand)
- Maltose/maltodextrin-binding periplasmic protein,adenylate cyclase exoy (84 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, alpha skeletal muscle (42 kDa, Protein from Rabbit)
- Magnesium ion (24 Da, Ligand)
- Phosphate ion (94 Da, Ligand)
Protective antigen pore translocating lethal factor N-terminal domain
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7kxr
Q-score: 0.315
Machen AJ, Fisher MT, Freudenthal BD
Sci Rep (2021) 11 pp. 13038-13038 [ DOI: doi:10.1038/s41598-021-91596-3 Pubmed: 34158520 ]
- Protective antigen (63 kDa, Protein from Bacillus anthracis)
- Anthrax toxin protective antigen translocating lethal factor n-terminal domain (Complex from Bacillus anthracis)
- Lethal factor (30 kDa, Protein from Bacillus anthracis)
- Calcium ion (40 Da, Ligand)
Anthrax toxin prepore in complex with the neutralizing Fab cAb29
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7o85
Q-score: 0.409
Hoelzgen F, Zalk R, Alcalay R, Cohen-Schwartz S, Garau G, Shahar A, Mazor O, Frank GA
Acta Crystallogr D Struct Biol (2021) 77 pp. 1197-1205 [ Pubmed: 34473089 DOI: doi:10.1107/S2059798321007816 ]
- Fab (Complex from Mus musculus)
- Fab (10 kDa, Protein from Mus musculus)
- Calcium ion (40 Da, Ligand)
- Fab bound to anthrax protective antigen (791 kDa, Complex)
- Protective antigen pa-63 (49 kDa, Protein from Bacillus anthracis)
- Anthrax protective antigen (Complex from Bacillus anthracis)
- Fab (10 kDa, Protein from Mus musculus)
Anthrax octamer prechannel bound to full-length edema factor
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6vra
Q-score: 0.435
Zhou K, Liu S, Hardenbrook NJ, Cui Y, Krantz BA, Zhou ZH
Structure (2020) 28 pp. 879-887.e3 [ Pubmed: 32521227 DOI: doi:10.1016/j.str.2020.05.009 ]
- Anthrax octamer prechannel bound to full-length edema factor (860 MDa, Complex from Bacillus anthracis)
- Protective antigen (82 kDa, Protein from Bacillus anthracis)
- Calcium ion (40 Da, Ligand)
- Protective antigen (82 kDa, Protein from Bacillus anthracis)
- Calmodulin-sensitive adenylate cyclase (88 kDa, Protein from Bacillus anthracis)
Anthrax octamer prechannel bound to full-length lethal factor
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6wjj
Q-score: 0.428
Zhou K, Liu S, Hardenbrook NJ, Cui Y, Krantz BA, Zhou ZH
Structure (2020) 28 pp. 879-887.e3 [ Pubmed: 32521227 DOI: doi:10.1016/j.str.2020.05.009 ]
- Protective antigen (82 kDa, Protein from Bacillus anthracis)
- Calcium ion (40 Da, Ligand)
- Protective antigen (82 kDa, Protein from Bacillus anthracis)
- Anthrax octamer prechannel bound to full-length lethal factor (852 MDa, Complex from Bacillus anthracis)
- Sulfate ion (96 Da, Ligand)
- Lethal factor (90 kDa, Protein from Bacillus anthracis)
- Zinc ion (65 Da, Ligand)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6zxj
Q-score: 0.396
Antoni C, Quentin D, Lang AE, Aktories K, Gatsogiannis C, Raunser S
PLoS Pathog (2020) 16 pp. e1008530-e1008530 [ DOI: doi:10.1371/journal.ppat.1008530 Pubmed: 32810181 ]
- Protective antigen (85 kDa, Protein from Bacillus anthracis)
- Lethal factor (93 kDa, Protein from Bacillus anthracis)
- Lethal factor (Complex from Bacillus anthracis)
- Fully-loaded anthrax lethal toxin in its heptameric pre-pore state (93 kDa, Complex)
- Protective antigen (Complex from Bacillus anthracis)