Cryo-EM structure of the DNA polymerase holoenzyme E9-A20-D4 of vaccinia virus
Burmeister WP, Boutin L, Balestra AC, Groeger H, Ballandras-Colas A, Hutin S, Kraft C, Grimm C, Boettcher B, Fischer U, Tarbouriech N, Iseni F
To Be Published
- E9-a20-d4 dna polymerase holoenzyme (196 MDa, Complex from Vaccinia virus)
- Dna polymerase (120 kDa, Protein from Vaccinia virus Copenhagen)
- Dna polymerase processivity factor component opg148 (49 kDa, Protein from Vaccinia virus Copenhagen)
- Uracil-dna glycosylase (27 kDa, Protein from Vaccinia virus Copenhagen)
Monkeypox virus DNA replication holoenzyme F8, A22 and E4 in complex with a DNA duplex and dCTP
Xu YX, Wu YQ, Wu XY, Zhang YY, Yang YX, Li DY, Yang B, Gao K, Zhang ZY, Dong CJ
To Be Published
- Dna polymerase (120 kDa, Protein from Monkeypox virus)
- Dna polymerase processivity factor component a20 (49 kDa, Protein from Monkeypox virus)
- E4r (27 kDa, Protein from Monkeypox virus)
- Dna (5'-d(p*gp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*gp*ap*tp*ap*ap*cp*tp*tp*ap*ap*tp*cp*tp*cp*ap*cp*ap*tp*ap*gp*cp*ap*gp*cp*t)-3') (11 kDa, DNA from DNA molecule)
- 2'-deoxycytidine-5'-triphosphate (467 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Dna (5'-d(p*ap*gp*cp*tp*gp*cp*tp*ap*tp*gp*ap*gp*ap*tp*tp*ap*ap*gp*tp*tp*ap*t)-3') (7 kDa, DNA from DNA molecule)
- Monkeypox virus replication holoenzyme f8-a22-e4 in complex with a dna duplex and dctp (191 MDa, Complex from Monkeypox virus)
Xu YX, Wu YQ, Wu XY, Zhang YY, Yang YX, Li DY, Yang B, Gao K, Zhang ZY, Dong CJ
To Be Published
- Dna polymerase (120 kDa, Protein from Monkeypox virus)
- Dna polymerase processivity factor component a20 (49 kDa, Protein from Monkeypox virus)
- Dna (5'-d(p*gp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*gp*ap*tp*ap*ap*cp*tp*tp*ap*ap*tp*cp*tp*cp*ap*cp*ap*tp*ap*gp*cp*ap*gp*cp*t)-3') (11 kDa, DNA from DNA molecule)
- Calcium ion (40 Da, Ligand)
- [(2s)-1-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3-oxidanyl-propan-2-yl]oxymethyl-[oxidanyl(phosphonooxy)phosphoryl]oxy-phosphinic acid (439 Da, Ligand)
- Dna (5'-d(p*ap*gp*cp*tp*gp*cp*tp*ap*tp*gp*ap*gp*ap*tp*tp*ap*ap*gp*tp*tp*ap*t)-3') (7 kDa, DNA from DNA molecule)
- Monkeypox virus replication holoenzyme f8-a22-e4 in complex with dna duplex and cidofovir diphosphate (191 MDa, Complex from Monkeypox virus)
- E4r (27 kDa, Protein from Monkeypox virus)
Monkeypox virus DNA replication holoenzyme F8, A22 and E4 complex in a DNA binding form
Xu YX, Wu YQ, Wu XY, Zhang YY, Yang YX, Li DY, Yang B, Gao K, Zhang ZY, Dong CJ
To Be Published
- Dna (5'-d(p*gp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*gp*ap*tp*ap*ap*cp*tp*tp*ap*ap*tp*cp*tp*cp*ap*cp*ap*tp*ap*gp*cp*ap*gp*cp*tp*)-3') (11 kDa, DNA from DNA molecule)
- Dna polymerase processivity factor component a20 (49 kDa, Protein from Monkeypox virus)
- E4r (27 kDa, Protein from Monkeypox virus)
- Dna (5'-d(p*ap*gp*cp*tp*gp*cp*tp*ap*tp*gp*tp*gp*ap*gp*ap*tp*tp*ap*ap*gp*tp*tp*ap*t)-3') (7 kDa, DNA from DNA molecule)
- Calcium ion (40 Da, Ligand)
- Monkeypox virus replication holoenzyme f8-a22-e4 complex with dna duplex only (191 MDa, Complex from Monkeypox virus)
- Dna polymerase (120 kDa, Protein from Monkeypox virus)
Human DNA polymerase theta helicase domain tetramer in the apo form
Zerio CJ, Bai Y, Sosa-Alvarado BA, Guzi T, Lander GC
To Be Published
- Dna polymerase theta (99 kDa, Protein from Homo sapiens)
- Human dna polymerase theta helicase domain (400 kDa, Complex from Homo sapiens)
Human DNA polymerase theta helicase domain dimer in the apo form
Zerio CJ, Bai Y, Sosa-Alvarado BA, Guzi T, Lander GC
To Be Published
- Dna polymerase theta (99 kDa, Protein from Homo sapiens)
- Human dna polymerase theta helicase domain (200 kDa, Complex from Homo sapiens)
Zerio CJ, Bai Y, Sosa-Alvarado BA, Guzi T, Lander GC
To Be Published
- Human dna polymerase theta helicase domain (Complex from Homo sapiens)
- Dna polymerase theta (99 kDa, Protein from Homo sapiens)
- Human dna polymerase theta helicase domain bound to stem-loop dna with microhomology in the 3' overhang (20 kDa, Complex from Homo sapiens)
- Stem-loop dna with microhomology in the 3' overhang (17 kDa, DNA from synthetic construct)
- Stem-loop dna with microhomology in the 3' overhang (Complex from Homo sapiens)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 9bh9
Q-score: 0.422
Zerio CJ, Bai Y, Sosa-Alvarado BA, Guzi T, Lander GC
To Be Published
- Human dna polymerase theta helicase domain (Complex from Homo sapiens)
- Dna polymerase theta (99 kDa, Protein from Homo sapiens)
- Human dna polymerase theta helicase domain bound to stem-loop dna with microhomology in the 3' overhang (20 kDa, Complex from Homo sapiens)
- Stem-loop dna with microhomology in the 3' overhang (17 kDa, DNA from synthetic construct)
- Stem-loop dna with microhomology in the 3' overhang (Complex from Homo sapiens)
Zerio CJ, Bai Y, Sosa-Alvarado BA, Guzi T, Lander GC
To Be Published
- Human dna polymerase theta helicase domain (Complex from Homo sapiens)
- Dna polymerase theta (99 kDa, Protein from Homo sapiens)
- Human dna polymerase theta helicase domain bound to stem-loop dna with microhomology in the 3' overhang (20 kDa, Complex from Homo sapiens)
- Stem-loop dna with microhomology in the 3' overhang (17 kDa, DNA from synthetic construct)
- Stem-loop dna with microhomology in the 3' overhang (Complex from Homo sapiens)
Cryo-EM structure of Pyrococcus furiosus transcription elongation complex bound to Spt4/5
Resolution: 3.45 Å
EM Method: Single-particle
Fitted PDBs: 8oki
Q-score: 0.48
Tarau DM, Grunberger F, Pilsl M, Reichelt R, Heiss FB, Koenig S, Urlaub H, Hausner W, Engel C, Grohmann D
Nucleic Acids Res (2024) [ ]
- Dna-directed rna polymerase subunit beta (127 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Transcription elongation factor spt4 (6 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Nucleotide strands (Complex from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo4 (14 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo7 (21 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Spt4/5 (Complex from Pyrococcus furiosus DSM 3638)
- Dna template strand (8 kDa, DNA from Pyrococcus furiosus DSM 3638)
- Zinc ion (65 Da, Ligand)
- Transcription elongation complex with spt4/5 (Complex)
- Dna-directed rna polymerase subunit rpo3 (31 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Rna (2 kDa, RNA from Pyrococcus furiosus DSM 3638)
- Dna non-template strand (7 kDa, DNA from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo1n (103 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Magnesium ion (24 Da, Ligand)
- Pyrococcus furiosus rna polymerase (Complex from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo10 (7 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo6 (6 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo5 (9 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo12 (5 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo1c (44 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Transcription elongation factor spt5 (16 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo11 (11 kDa, Protein from Pyrococcus furiosus DSM 3638)