The structure of EBOV L-VP35-RNA complex
Resolution: 2.95 Å
EM Method: Single-particle
Fitted PDBs: 8jsl
Q-score: 0.547
Peng Q, Yuan B, Cheng J, Wang M, Gao S, Bai S, Zhao X, Qi J, Gao GF, Shi Y
Nature (2023) 622 pp. 603-610 [ Pubmed: 37699521 DOI: doi:10.1038/s41586-023-06608-1 ]
- Polymerase cofactor vp35 (37 kDa, Protein from Ebola virus)
- The leader sequence of ebov (5 kDa, RNA from Ebola virus)
- Rna-directed rna polymerase l (252 kDa, Protein from Ebola virus)
- The structure of ebov l-vp35-rna complex (Complex from Ebola virus)
- Zinc ion (65 Da, Ligand)
The structure of EBOV L-VP35-RNA complex (conformation 1)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8jsm
Q-score: 0.495
Peng Q, Yuan B, Cheng J, Wang M, Gao S, Bai S, Zhao X, Qi J, Gao GF, Shi Y
Nature (2023) 622 pp. 603-610 [ Pubmed: 37699521 DOI: doi:10.1038/s41586-023-06608-1 ]
- The leader sequence of ebov genome. (5 kDa, RNA from Ebola virus)
- Polymerase cofactor vp35 (37 kDa, Protein from Ebola virus)
- Rna-directed rna polymerase l (252 kDa, Protein from Ebola virus)
- The structure of ebov l-vp35-rna complex (conformation 1) (Complex from Ebola virus)
- Zinc ion (65 Da, Ligand)
The structure of EBOV L-VP35-RNA complex (conformation 2)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8jsn
Q-score: 0.475
Peng Q, Yuan B, Cheng J, Wang M, Gao S, Bai S, Zhao X, Qi J, Gao GF, Shi Y
Nature (2023) 622 pp. 603-610 [ Pubmed: 37699521 DOI: doi:10.1038/s41586-023-06608-1 ]
- Polymerase cofactor vp35 (37 kDa, Protein from Ebola virus)
- The leader sequence of ebov genome (5 kDa, RNA from Ebola virus)
- Rna-directed rna polymerase l (252 kDa, Protein from Ebola virus)
- The structure of ebov l-vp35-rna complex (conformation 2) (Complex from Ebola virus)
- Zinc ion (65 Da, Ligand)
Structure of EBOV GP lacking the mucin-like domain with 2.1.1D5 scFv and 6D6 scFv bound
Resolution: 2.53 Å
EM Method: Single-particle
Fitted PDBs: 8dpl
Q-score: 0.536
Yu X, Hastie KM, Davis CW, Avalos RD, Williams D, Parekh D, Hui S, Mann C, Hariharan C, Takada A, Ahmed R, Saphire EO
Cell Rep (2023) 42 pp. 113366-113366 [ Pubmed: 37938974 DOI: doi:10.1016/j.celrep.2023.113366 ]
- 6d6 single-chain variable fragment (25 kDa, Protein from Mus musculus)
- Glycoprotein gp1 (31 kDa, Protein from Ebola virus - Mayinga, Zaire, 1976)
- 2.1.1d5 light chain variable domain (11 kDa, Protein from Homo sapiens)
- Glycoprotein gp2 (15 kDa, Protein from Ebola virus - Mayinga, Zaire, 1976)
- Structure of ebov gp in complex with 2.1.1d5 scfv and 6d6 scfv (Complex from Ebola virus - Mayinga, Zaire, 1976)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 2.1.1d5 heavy chain variable domain (13 kDa, Protein from Homo sapiens)
Structure of EBOV GP lacking the mucin-like domain with 9.20.1A2 Fab and 6D6 scFv bound
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8dpm
Q-score: 0.505
Yu X, Hastie KM, Davis CW, Avalos RD, Williams D, Parekh D, Hui S, Mann C, Hariharan C, Takada A, Ahmed R, Saphire EO
Cell Rep (2023) 42 pp. 113366-113366 [ Pubmed: 37938974 DOI: doi:10.1016/j.celrep.2023.113366 ]
- Antibody 6d6 scfv (25 kDa, Protein from Mus musculus)
- Antibody 9.20.1a2 fab heavy chain (13 kDa, Protein from Homo sapiens)
- Glycoprotein gp1 (31 kDa, Protein from Ebola virus - Mayinga, Zaire, 1976)
- Glycoprotein gp2 (15 kDa, Protein from Ebola virus - Mayinga, Zaire, 1976)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Complex of ebola virus glycoprotein trimer with 1a2 antibody fab and 6d6 antibody scfv (Complex from Ebola virus - Mayinga, Zaire, 1976)
- Antibody 9.20.1a2 fab (Complex from Homo sapiens)
- Antibody 9.20.1a2 fab light chain (11 kDa, Protein from Homo sapiens)
Tomogram of an Ebola VLP composed of GP, VP40, NP, VP24 and VP35 at pH 7.4 (Figure 1A-D)
Winter SL, Golani G, Lolicato F, Vallbracht M, Thiyagarajah K, Ahmed SS, Luchtenborg C, Fackler OT, Brugger B, Hoenen T, Nickel W, Schwarz US, Chlanda P
EMBO J (2023) 42 pp. e113578-e113578 [ DOI: doi:10.15252/embj.2023113578 Pubmed: 37082863 ]
Tomogram of an Ebola VLP composed of VP40 at pH 4.5 (Figure 1J)
Winter SL, Golani G, Lolicato F, Vallbracht M, Thiyagarajah K, Ahmed SS, Luchtenborg C, Fackler OT, Brugger B, Hoenen T, Nickel W, Schwarz US, Chlanda P
EMBO J (2023) 42 pp. e113578-e113578 [ DOI: doi:10.15252/embj.2023113578 Pubmed: 37082863 ]
Tomogram of an EBOV-infected Huh7 cell showing a late endosome with internalized EBOV particles
Winter SL, Golani G, Lolicato F, Vallbracht M, Thiyagarajah K, Ahmed SS, Luchtenborg C, Fackler OT, Brugger B, Hoenen T, Nickel W, Schwarz US, Chlanda P
EMBO J (2023) 42 pp. e113578-e113578 [ DOI: doi:10.15252/embj.2023113578 Pubmed: 37082863 ]
Tomogram of an extracellular EBOV particle adjacent to an EBOV-infected Huh7 cell
Winter SL, Golani G, Lolicato F, Vallbracht M, Thiyagarajah K, Ahmed SS, Luchtenborg C, Fackler OT, Brugger B, Hoenen T, Nickel W, Schwarz US, Chlanda P
EMBO J (2023) 42 pp. e113578-e113578 [ DOI: doi:10.15252/embj.2023113578 Pubmed: 37082863 ]
Lloviu cuevavirus nucleoprotein-RNA complex
Resolution: 3.0356 Å
EM Method: Single-particle
Fitted PDBs: 7ypw
Q-score: 0.407
Hu S, Fujita-Fujiharu Y, Sugita Y, Wendt L, Muramoto Y, Nakano M, Hoenen T, Noda T
Pnas Nexus (2023) 2 pp. pgad120-pgad120 [ DOI: doi:10.1093/pnasnexus/pgad120 Pubmed: 37124400 ]
- Lloviu cuevavirus nucleoprotein rna complex (Complex from Lloviu cuevavirus)
- Nucleoprotein (84 kDa, Protein from Lloviu cuevavirus)
- Rna (5'-r(p*up*up*up*up*up*u)-3') (1 kDa, RNA from synthetic construct)