Saccharomyces cerevisiae FAS type I
Henderikx RJM, Mann D, Domanska A, Dong J, Shahzad S, Lak B, Filopoulou A, Ludig D, Grininger M, Momoh J, Laanto E, Oksanen HM, Bisikalo K, Williams PA, Butcher SJ, Peters PJ, Beulen BWAMM
Acta Crystallogr D Struct Biol (2024) 80 pp. 232-246 [ Pubmed: 38488730 DOI: doi:10.1107/S2059798324001852 ]
MBP-tagged fusion fatty acid synthase from S. cerevisiae
Samani EK, Chen AC, Lou JW, Dai DL, Keszei AFA, Tan G, Boone C, Grininger M, Mazhab-Jafari MT
Commun Biol (2024) 7 pp. 92- [ DOI: doi:10.1038/s42003-024-05777-7 ]
MBP tagged and Mixed chain fatty acid synthase, FAS2_deltaACP
Samani EK, Chen AC, Lou JW, Dai DL, Keszei AFA, Tan G, Boone C, Grininger M, Mazhab-Jafari MT
Commun Biol (2024) 7 pp. 92- [ DOI: doi:10.1038/s42003-024-05777-7 ]
MBP tagged mixed chain FAS, fusFAS_deltaACP
Samani EK, Chen AC, Lou JW, Dai DL, Keszei AFA, Tan G, Boone C, Grininger M, Mazhab-Jafari MT
Commun Biol (2024) 7 pp. 92- [ DOI: doi:10.1038/s42003-024-05777-7 ]
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8prv
Q-score: 0.581
Singh K, Bunzel G, Graf B, Yip KM, Neumann-Schaal M, Stark H, Chari A
Cell (2023) 186 pp. 5054-5067.e16 [ Pubmed: 37949058 DOI: doi:10.1016/j.cell.2023.10.009 ]
- Fatty acid synthase subunit alpha (207 kDa, Protein from Saccharomyces cerevisiae)
- Yeast fatty acid synthase (Complex from Saccharomyces cerevisiae)
- Coenzyme a (767 Da, Ligand)
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] 3-oxidanylidenebutanethioate (442 Da, Ligand)
- Fatty acid synthase subunit beta (229 kDa, Protein from Saccharomyces cerevisiae)
- Flavin mononucleotide (456 Da, Ligand)
- Nadp nicotinamide-adenine-dinucleotide phosphate (743 Da, Ligand)
Cryo-EM structure of the yeast fatty acid synthase at 1.9 angstrom resolution
Singh K, Bunzel G, Graf B, Yip KM, Neumann-Schaal M, Stark H, Chari A
Cell (2023) 186 pp. 5054-5067.e16 [ Pubmed: 37949058 DOI: doi:10.1016/j.cell.2023.10.009 ]
- Fatty acid synthase subunit alpha (207 kDa, Protein from Saccharomyces cerevisiae)
- Yeast fatty acid synthase (Complex from Saccharomyces cerevisiae)
- Coenzyme a (767 Da, Ligand)
- Water (18 Da, Ligand)
- 1-deoxy-1-(7,8-dimethyl-2,4-dioxo-3,4-dihydro-2h-benzo[g]pteridin-1-id-10(5h)-yl)-5-o-phosphonato-d-ribitol (458 Da, Ligand)
- Fatty acid synthase subunit beta (229 kDa, Protein from Saccharomyces cerevisiae)
- Nadp nicotinamide-adenine-dinucleotide phosphate (743 Da, Ligand)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8ps1
Q-score: 0.588
Singh K, Bunzel G, Graf B, Yip KM, Neumann-Schaal M, Stark H, Chari A
Cell (2023) 186 pp. 5054-5067.e16 [ Pubmed: 37949058 DOI: doi:10.1016/j.cell.2023.10.009 ]
- Fatty acid synthase subunit alpha (207 kDa, Protein from Saccharomyces cerevisiae)
- Yeast fatty acid synthase (Complex from Saccharomyces cerevisiae)
- Coenzyme a (767 Da, Ligand)
- Fatty acid synthase subunit beta (229 kDa, Protein from Saccharomyces cerevisiae)
- Flavin mononucleotide (456 Da, Ligand)
- Nadp nicotinamide-adenine-dinucleotide phosphate (743 Da, Ligand)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8ps2
Q-score: 0.575
Singh K, Bunzel G, Graf B, Yip KM, Neumann-Schaal M, Stark H, Chari A
Cell (2023) 186 pp. 5054-5067.e16 [ Pubmed: 37949058 DOI: doi:10.1016/j.cell.2023.10.009 ]
- Fatty acid synthase subunit alpha (207 kDa, Protein from Saccharomyces cerevisiae)
- Fatty acid synthase subunit beta (228 kDa, Protein from Saccharomyces cerevisiae)
- Malonyl-coenzyme a (853 Da, Ligand)
- Yeast fatty acid synthase (Complex from Saccharomyces cerevisiae)
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (~{e})-but-2-enethioate (426 Da, Ligand)
- Flavin mononucleotide (456 Da, Ligand)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 8ps8
Q-score: 0.38
Singh K, Bunzel G, Graf B, Yip KM, Neumann-Schaal M, Stark H, Chari A
Cell (2023) 186 pp. 5054-5067.e16 [ Pubmed: 37949058 DOI: doi:10.1016/j.cell.2023.10.009 ]
- Fatty acid synthase subunit beta (228 kDa, Protein from Saccharomyces cerevisiae)
- Yeast fatty acid synthase (Complex from Saccharomyces cerevisiae)
- Fatty acid synthase subunit alpha (207 kDa, Protein from Saccharomyces cerevisiae)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8ps9
Q-score: 0.574
Singh K, Bunzel G, Graf B, Yip KM, Neumann-Schaal M, Stark H, Chari A
Cell (2023) 186 pp. 5054-5067.e16 [ Pubmed: 37949058 DOI: doi:10.1016/j.cell.2023.10.009 ]
- Fatty acid synthase subunit alpha (207 kDa, Protein from Saccharomyces cerevisiae)
- Fatty acid synthase subunit beta (228 kDa, Protein from Saccharomyces cerevisiae)
- Malonyl-coenzyme a (853 Da, Ligand)
- Yeast fatty acid synthase (Complex from Saccharomyces cerevisiae)
- Flavin mononucleotide (456 Da, Ligand)