Structure of the native microtubule lattice nucleated from the yeast spindle pole body
Resolution: 6.6 Å
EM Method: Subtomogram averaging
Fitted PDBs: 8qv0
Q-score: 0.123
Dendooven T, Yatskevich S, Burt A, Chen ZA, Bellini D, Rappsilber J, Kilmartin JV, Barford D
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01281-y Pubmed: 38609662 ]
Resolution: 9.2 Å
EM Method: Subtomogram averaging
Fitted PDBs: 8qv2
Q-score: 0.112
Dendooven T, Yatskevich S, Burt A, Chen ZA, Bellini D, Rappsilber J, Kilmartin JV, Barford D
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01281-y Pubmed: 38609662 ]
- Water (18 Da, Ligand)
- Tubulin gamma chain (52 kDa, Protein from Saccharomyces cerevisiae)
- Unkown protein (5 kDa, Protein from Saccharomyces cerevisiae)
- Spindle pole body component 110 (111 kDa, Protein from Saccharomyces cerevisiae)
- Spindle pole body component (96 kDa, Protein from Saccharomyces cerevisiae)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin beta chain (50 kDa, Protein from Saccharomyces cerevisiae)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Spindle pole body component (98 kDa, Protein from Saccharomyces cerevisiae)
- Tubulin alpha-1 chain (49 kDa, Protein from Saccharomyces cerevisiae)
- Y-tubulin ring complex capping the microtubule minus end (Cellular component from Saccharomyces cerevisiae)
Resolution: 8.2 Å
EM Method: Subtomogram averaging
Fitted PDBs: 8qv3
Q-score: 0.144
Dendooven T, Yatskevich S, Burt A, Chen ZA, Bellini D, Rappsilber J, Kilmartin JV, Barford D
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01281-y Pubmed: 38609662 ]
- Water (18 Da, Ligand)
- Unknown protein (5 kDa, Protein from Saccharomyces cerevisiae)
- Y-tubulin small complex bound to two alpha/beta tubulin dimers (Cellular component from Saccharomyces cerevisiae)
- Spindle pole body component 110 (111 kDa, Protein from Saccharomyces cerevisiae)
- Spindle pole body component (96 kDa, Protein from Saccharomyces cerevisiae)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin beta chain (50 kDa, Protein from Saccharomyces cerevisiae)
- Spindle pole body component (98 kDa, Protein from Saccharomyces cerevisiae)
- Tubulin alpha-1 chain (49 kDa, Protein from Saccharomyces cerevisiae)
- Tubulin gamma chain (52 kDa, Protein from Saccharomyces cerevisiae)
- Unknown protein (5 kDa, Protein from Saccharomyces cerevisiae)
in situ subtomogram average of C. elegans microtubules in mitotic centrosomes
Tollervey F, Rios MU, Zagoriy E, Woodruff JB, Mahamid J
bioRxiv (2024) [ DOI: doi:10.1101/2024.04.03.587742 Pubmed: 38617234 ]
in-situ subtomogram average of C. elegans centrioles in centrosomes
Tollervey F, Rios MU, Zagoriy E, Woodruff JB, Mahamid J
bioRxiv (2024) [ DOI: doi:10.1101/2024.04.03.587742 Pubmed: 38617234 ]
in situ subtomogram average of C. elegans gamma-tubulin ring complexes in mitotic centrosomes
Tollervey F, Rios MU, Zagoriy E, Woodruff JB, Mahamid J
bioRxiv (2024) [ DOI: doi:10.1101/2024.04.03.587742 Pubmed: 38617234 ]
Cryo-ET of a mitotic centrosome in an embryonic C. elegans cell
Tollervey F, Rios MU, Zagoriy E, Woodruff JB, Mahamid J
bioRxiv (2024) [ DOI: doi:10.1101/2024.04.03.587742 Pubmed: 38617234 ]
Vertebrate microtubule-capping gamma-tubulin ring complex
Resolution: 17.0 Å
EM Method: Single-particle
Fitted PDBs: 9eoj
Q-score: 0.056
Vermeulen BJ, Bohler A, Gao Q, Neuner A, Zupa E, Chu Z, Wurtz M, Jakle U, Gruss OJ, Pfeffer S, Schiebel E
EMBO J (2024) [ Pubmed: 38600243 DOI: doi:10.1038/s44318-024-00087-4 ]
- Gamma-tubulin complex component (76 kDa, Protein from Xenopus laevis)
- Gamma-tubulin complex component (103 kDa, Protein from Xenopus laevis)
- Vertebrate microtubule-capping gamma-tubulin ring complex (Complex from Xenopus laevis)
- Tubulin gamma-1 chain (51 kDa, Protein from Xenopus laevis)
- Gamma-tubulin complex component 3 homolog (103 kDa, Protein from Xenopus laevis)
- Gamma-tubulin complex component 6 (193 kDa, Protein from Xenopus laevis)
- Gamma-tubulin complex component (117 kDa, Protein from Xenopus laevis)
- Mitotic-spindle organizing protein 1 (7 kDa, Protein from Xenopus laevis)
Outer kinetochore Ndc80-Dam1 alpha/beta-tubulin complex
Resolution: 3.54 Å
EM Method: Single-particle
Fitted PDBs: 8qau
Q-score: 0.343
Muir KW, Batters C, Dendooven T, Yang J, Zhang Z, Burt A, Barford D
bioRxiv (2023) [ DOI: doi:10.1101/2023.07.20.549907 ]
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1a chain (50 kDa, Protein from Sus scrofa)
- Kinetochore protein ndc80 (80 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dam1 (38 kDa, Protein from Saccharomyces cerevisiae)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Kinetochore protein nuf2 (53 kDa, Protein from Saccharomyces cerevisiae)
- Outer kinetochore ndc80-nuf2 ch domains bound to dam1 in complex with alpha-beta tubulin (Complex from Saccharomyces cerevisiae)
- Taxol (853 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)