Cryo-EM structure of Pyrococcus furiosus transcription elongation complex
Tarau DM, Grunberger F, Pilsl M, Reichelt R, Heiss FB, Koenig S, Urlaub H, Hausner W, Engel C, Grohmann D
Nucleic Acids Res (2024) [ ]
- Nucleotide strands (Complex from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo12 (5 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo5 (9 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo6 (6 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Rna (2 kDa, RNA from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo7 (21 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit beta (127 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo10 (7 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Transcription elongation complex (tec) (Complex)
- Pyrococcus furiosus rna polymerase (Complex from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo1c (44 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo4 (14 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Dna non-template strand (2 kDa, DNA from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo1n (103 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Magnesium ion (24 Da, Ligand)
- Dna template strand (6 kDa, DNA from Pyrococcus furiosus DSM 3638)
- Dna-directed rna polymerase subunit rpo3 (29 kDa, Protein from Pyrococcus furiosus DSM 3638)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit rpo11 (11 kDa, Protein from Pyrococcus furiosus DSM 3638)
RNA polymerase II core initially transcribing complex with an ordered RNA of 8 nt
Zhan Y, Grabbe F, Oberbeckmann E, Dienemann C, Cramer P
To Be Published
- Transcription initiation factor iib (34 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit e (24 kDa, Protein from Sus scrofa)
- General transcription factor iie subunit 1 (49 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Sus scrofa)
- Rna polymerase ii subunit d (20 kDa, Protein from Sus scrofa)
- Transcription initiation factor iia subunit 1 (41 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Sus scrofa)
- Rna polymerase ii subunit k (7 kDa, Protein from Sus scrofa)
- Dna and rna (Complex from unidentified adenovirus)
- Dna-directed rna polymerase subunit (217 kDa, Protein from Sus scrofa)
- Tata-box-binding protein, transcription initiation factor iia (Complex from Homo sapiens)
- Transcription initiation factor iib, iie and iif (Complex from Homo sapiens)
- Tata-box-binding protein (37 kDa, Protein from Homo sapiens)
- General transcription factor iif subunit 1 (58 kDa, Protein from Homo sapiens)
- Rna (2 kDa, RNA from unidentified adenovirus)
- Template dna (42 kDa, DNA from unidentified adenovirus)
- Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Sus scrofa)
- Core initially transcribing complex with an ordered rna of 8 nt (Complex)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Sus scrofa)
- Non-template dna (43 kDa, DNA from unidentified adenovirus)
- Rna polymerase ii (Complex from Sus scrofa)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Transcription initiation factor iia subunit 2 (12 kDa, Protein from Homo sapiens)
- Transcription initiation factor iie subunit beta (33 kDa, Protein from Homo sapiens)
- General transcription factor iif subunit 2 (28 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerase subunit beta (147 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Sus scrofa)
RNA polymerase II core initially transcribing complex with an ordered RNA of 10 nt
Zhan Y, Grabbe F, Oberbeckmann E, Dienemann C, Cramer P
To Be Published
- Transcription initiation factor iib (34 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit e (24 kDa, Protein from Sus scrofa)
- General transcription factor iie subunit 1 (49 kDa, Protein from Homo sapiens)
- Core initially transcribing complex with an ordered rna of 10 nt (Complex)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Sus scrofa)
- Transcription initiation factor iia (Complex from Homo sapiens)
- Rna polymerase ii subunit d (20 kDa, Protein from Sus scrofa)
- Transcription initiation factor iia subunit 1 (41 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Sus scrofa)
- Rna polymerase ii subunit k (7 kDa, Protein from Sus scrofa)
- Dna and rna (Complex from unidentified adenovirus)
- Tata-box-binding protein (37 kDa, Protein from Homo sapiens)
- General transcription factor iif subunit 1 (58 kDa, Protein from Homo sapiens)
- Template dna (42 kDa, DNA from unidentified adenovirus)
- Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerase subunit (218 kDa, Protein from Sus scrofa)
- Rna (3 kDa, RNA from unidentified adenovirus)
- Tata-box-binding protein, transcription initiation factor iib, iie and iif (Complex from Homo sapiens)
- Non-template dna (43 kDa, DNA from unidentified adenovirus)
- Rna polymerase ii (Complex from Sus scrofa)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Transcription initiation factor iia subunit 2 (12 kDa, Protein from Homo sapiens)
- Transcription initiation factor iie subunit beta (33 kDa, Protein from Homo sapiens)
- General transcription factor iif subunit 2 (28 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerase subunit beta (147 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Sus scrofa)
RNA polymerase II early elongation complex bound to TFIIE and TFIIF - state b (composite structure)
Zhan Y, Grabber F, Oberbeckmann E, Dienemann C, Cramer P
To Be Published
- Dna-directed rna polymerase ii subunit e (24 kDa, Protein from Sus scrofa)
- Rna (4 kDa, RNA from unidentified adenovirus)
- General transcription factor iie subunit 1 (49 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Sus scrofa)
- Rna polymerase ii subunit d (20 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Sus scrofa)
- Rna polymerase ii subunit k (7 kDa, Protein from Sus scrofa)
- Dna and rna (Complex from unidentified adenovirus)
- Rna polymerase ii early elongation complex bound to tfiie and tfiif - state b (Complex)
- Transcription factor iie subunit 1 and transcription factor iif (Complex from Homo sapiens)
- General transcription factor iif subunit 1 (58 kDa, Protein from Homo sapiens)
- Template dna (42 kDa, DNA from unidentified adenovirus)
- Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerase subunit (218 kDa, Protein from Sus scrofa)
- Non-template dna (43 kDa, DNA from unidentified adenovirus)
- Rna polymerase ii (Complex from Sus scrofa)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- General transcription factor iif subunit 2 (28 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerase subunit beta (147 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Sus scrofa)
RNA polymerase II early elongation complex bound to TFIIE and TFIIF - state a (composite structure)
Zhan Y, Grabbe F, Oberbeckmann E, Dienemann C, Cramer P
To Be Published
- Dna-directed rna polymerase ii subunit e (24 kDa, Protein from Sus scrofa)
- Rna (4 kDa, RNA from unidentified adenovirus)
- Transcription factor iie and iif (Cell from Homo sapiens)
- General transcription factor iie subunit 1 (49 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Sus scrofa)
- Rna polymerase ii subunit d (20 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Sus scrofa)
- Rna polymerase ii subunit k (7 kDa, Protein from Sus scrofa)
- Dna and rna (Complex from unidentified adenovirus)
- General transcription factor iif subunit 1 (58 kDa, Protein from Homo sapiens)
- Template dna (42 kDa, DNA from unidentified adenovirus)
- Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerase subunit (218 kDa, Protein from Sus scrofa)
- Non-template dna (43 kDa, DNA from unidentified adenovirus)
- Rna polymerase ii (Complex from Sus scrofa)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Sus scrofa)
- Rna polymerase ii early elongation complex bound to tfiie and tfiif - state a (Complex)
- Magnesium ion (24 Da, Ligand)
- Transcription initiation factor iie subunit beta (33 kDa, Protein from Homo sapiens)
- General transcription factor iif subunit 2 (28 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerase subunit beta (147 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Sus scrofa)
Structure of the Mumps Virus L Protein Bound by Phosphoprotein Tetramer (composite map)
To Be Published
- Rna-directed rna polymerase l (256 kDa, Protein from Mumps orthorubulavirus)
- Phosphoprotein (41 kDa, Protein from Mumps orthorubulavirus)
- The muv polymerase complex of rna-directed rna polymerase l with tetrameric phosphoproteins (Complex from Mumps orthorubulavirus)
- Zinc ion (65 Da, Ligand)
Structure of the Mumps Virus L Protein (state2) Bound by Phosphoprotein Tetramer
To Be Published
- Rna-directed rna polymerase l (256 kDa, Protein from Mumps virus strain Jeryl Lynn)
- The muv polymerase complex of rna-directed rna polymerase l with tetrameric phosphoproteins (Complex from Mumps orthorubulavirus)
- Phosphoprotein (41 kDa, Protein from Mumps virus strain Jeryl Lynn)
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of the human mPSF with PAPOA C-terminus peptide (PAPOAc)
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 8r8r
Q-score: 0.578
Todesca S, Sandmeir F, Keidel A, Conti E
RNA (2024) [ DOI: doi:10.1261/rna.079915.123 Pubmed: 38538052 ]
- Cleavage and polyadenylation specificity factor subunit 4 (31 kDa, Protein from Homo sapiens)
- Pre-mrna 3' end processing protein wdr33 (47 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Cleavage and polyadenylation specificity factor subunit 1 (161 kDa, Protein from Homo sapiens)
- Cdna flj50397, highly similar to poly(a) polymerase alpha (2 kDa, Protein from Homo sapiens)
- Rna (5'-r(p*ap*ap*up*ap*ap*a)-3') (1 kDa, RNA from Homo sapiens)
- Mpsf with papoa c-terminus peptide (papoac) (Complex from Homo sapiens)