Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8wa2
Q-score: 0.457
Huang J, Tao H, Chen J, Shen Y, Lei J, Pan J, Yan C, Yan N
Cell (2024) [ DOI: doi:10.1016/j.cell.2024.02.037 Pubmed: 38552612 ]
- Mstax (6 kDa, Protein from synthetic construct)
- Calcium ion (40 Da, Ligand)
- Alpha-d-galactopyranose (180 Da, Ligand)
- Alpha-l-arabinofuranose (150 Da, Ligand)
- Mastigoneme (Cellular component from Chlamydomonas reinhardtii)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Mst1 (206 kDa, Protein from Chlamydomonas reinhardtii)
Chlamydomonas reinhardtii mastigoneme (constituent map 1)
Dai J, Ma M, Niu Q, Eisert RJ, Wang X, Das P, Lechtreck KF, Dutcher SK, Zhang R, Brown A
Cell (2024) 187 pp. 1907-1921.e16 [ Pubmed: 38552624 DOI: doi:10.1016/j.cell.2024.03.005 ]
Chlamydomonas reinhardtii mastigoneme (constituent map 2)
Dai J, Ma M, Niu Q, Eisert RJ, Wang X, Das P, Lechtreck KF, Dutcher SK, Zhang R, Brown A
Cell (2024) 187 pp. 1907-1921.e16 [ Pubmed: 38552624 DOI: doi:10.1016/j.cell.2024.03.005 ]
Chlamydomonas reinhardtii mastigoneme (constituent map 3)
Dai J, Ma M, Niu Q, Eisert RJ, Wang X, Das P, Lechtreck KF, Dutcher SK, Zhang R, Brown A
Cell (2024) 187 pp. 1907-1921.e16 [ Pubmed: 38552624 DOI: doi:10.1016/j.cell.2024.03.005 ]
Composite cryo-EM map of the Chlamydomonas reinhardtii mastigoneme
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 9b4h
Q-score: 0.488
Dai J, Ma M, Niu Q, Eisert RJ, Wang X, Das P, Lechtreck KF, Dutcher SK, Zhang R, Brown A
Cell (2024) 187 pp. 1907-1921.e16 [ Pubmed: 38552624 DOI: doi:10.1016/j.cell.2024.03.005 ]
In situ cryo-ET dataset of Chlamydomonas reinhardtii prepared using cryo-plasmaFIB milling
Khavnekar S, Kelley R, Waltz F, Wietrzynski W, Zhang X, Obr M, Tagiltsev G, Beck F, Wan W, Briggs J, Engel B, Plitzko J, Kotecha A
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada (2023) 29 pp. 961-963 [ Pubmed: 37613825 DOI: 10.1093/micmic/ozad067.480 ]
Image sets:
In situ nucleosome subtomogram average from Chlamydomonas reinhardtii
To Be Published
Cryo-EM Structure of native Photosystem II assembly intermediate fromChlamydomonas reinhardtii
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8r2i
Q-score: 0.533
Fadeeva M, Klaiman D, Kandiah E, Nelson N
Front Plant Sci (2023) 14 pp. 1334608-1334608 [ Pubmed: 38322422 DOI: doi:10.3389/fpls.2023.1334608 ]
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- Beta-carotene (536 Da, Ligand)
- Photosystem ii reaction center protein h (7 kDa, Protein from Chlamydomonas reinhardtii)
- Photosystem ii assembly intermediate from chlamydomonas reinhardtii (Complex from Chlamydomonas reinhardtii)
- Cytochrome b559 subunit alpha (8 kDa, Protein from Chlamydomonas reinhardtii)
- Chain u, predicted protein (11 kDa, Protein from Chlamydomonas reinhardtii)
- Photosystem ii protein d1 (36 kDa, Protein from Chlamydomonas reinhardtii)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Photosystem ii reaction center protein k (4 kDa, Protein from Chlamydomonas reinhardtii)
- Water (18 Da, Ligand)
- Octyl beta-d-glucopyranoside (292 Da, Ligand)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Photosystem ii cp43 reaction center protein (46 kDa, Protein from Chlamydomonas reinhardtii)
- 2,3-dimethyl-5-(3,7,11,15,19,23,27,31,35-nonamethyl-2,6,10,14,18,22,26,30,34-hexatriacontanonaenyl-2,5-cyclohexadiene-1,4-dione-2,3-dimethyl-5-solanesyl-1,4-benzoquinone (749 Da, Ligand)
- Pheophytin a (871 Da, Ligand)
- Photosystem ii reaction center protein i (3 kDa, Protein from Chlamydomonas reinhardtii)
- Cytochrome b559 subunit beta (3 kDa, Protein from Chlamydomonas reinhardtii)
- Chlorophyll a (893 Da, Ligand)
- Bicarbonate ion (61 Da, Ligand)
- Fe (ii) ion (55 Da, Ligand)
- Photosystem ii d2 protein (39 kDa, Protein from Chlamydomonas reinhardtii)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Photosystem ii cp47 reaction center protein (53 kDa, Protein from Chlamydomonas reinhardtii)
- Photosystem ii reaction center protein ycf12 (3 kDa, Protein from Chlamydomonas reinhardtii)
Khavnekar S, Kelley R, Waltz F, Wietrzynski W, Zhang X, Obr M, Tagiltsev G, Beck F, Wan W, Briggs J, Engel B, Plitzko J, Kotecha A
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada (2023) 29 pp. 961-963 [ Pubmed: 37613825 DOI: 10.1093/micmic/ozad067.480 ]
Image sets:
Characterization of the Chlamydomonas Flagellar Mastigoneme Filament Structure at 3.6A
Resolution: 3.62 Å
EM Method: Single-particle
Fitted PDBs: 8tx1
Q-score: 0.22
Wang Y, Yang J, Hu F, Yang Y, Huang K, Zhang K
J Cell Biol (2023) 222 [ Pubmed: 37882754 DOI: doi:10.1083/jcb.202301066 ]
- Mastigoneme (Cellular component from Chlamydomonas reinhardtii)
- Mastigoneme-like protein (205 kDa, Protein from Chlamydomonas reinhardtii)